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. 2017 Aug 21;7:9018. doi: 10.1038/s41598-017-09432-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PNG+aFGF+ChABC treatment maintains locomotor and improves micturition patterns. (a) Locomotor BBB score of rats prior to the repair surgery (Pre (8)) and following the repair surgery (weeks 9–40). n = 6 per group except at week 9 n = 5 for PNG and aFGF+ChABC. Between groups: ^&p = 0.0640, ^$p = 0.0931, ^%p = 0.0816, ^#p = 0.0794, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 Two-way repeated measures ANOVA Fisher’s Least Significant Difference post-hoc test. Within group ^δp = 0.0582, *p < 0.05, ***p < 0.001, ****p < 0.0001 One-way ANOVA, Fisher’s Least Significant Difference post-hoc test. (b) Representative smoothed metabolic cage traces 40 weeks after injury. (c,d) Metabolic cage quantification of average void frequency (c) and void volume (d). n = 6 per group. Between groups: **p < 0.01. Two-way repeated measures ANOVA, Fisher’s Least Significant Difference post-hoc test. Within group *p < 0.05 One-way ANOVA, Fisher’s Least Significant Difference post-hoc test. Data represent mean ± S.E.M.