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. 2017 Aug 21;7:9022. doi: 10.1038/s41598-017-08422-y

Figure 1.

Figure 1

Effect of ISL on cell proliferation and apoptosis in breast cancer cells. (A) Cell viability of MCF-7 and MDA-MB-231 after 24 h, 48 h, 72 h ISL treatment. (B) Representative images of induction of apoptosis in breast cancer cells determined by Flow cytometry analysis cultured with ISL (12.5, 25, 50 μM) for 24 h. (C) Percentages of early apoptotic cells and late apoptotic cells in different dose of ISL intervention according to Flow cytometry assay analysis. (D,F) Western blot analysis of Bcl-2, Bax, Cleaved Caspase-9, in MCF-7 and MDA-MB-231 after 24 h ISL treatment. The full-length blots were presented in the Supplementary Fig. 7. (E) Western blot analysis of Cyt C release in breast cancer cells after 24 h ISL interference. The full-length blots were presented in the Supplementary Fig. 8. Data represent the mean ± s.d. *P < 0.05, **P < 0.01.