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. 2017 Aug 21;7:9022. doi: 10.1038/s41598-017-08422-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Deletion of PTEN expression restricts the anti-cancer effect of ISL on breast cancer cells. (A) qRT-PCR analysis of PTEN mRNA expression in siRNA-NC and siRNA PTEN transfection groups after 24 h ISL treatment. (B) qRT-PCR analysis of BAX mRNA expression in siRNA-NC and siRNA PTEN transfection groups with ISL interference. (C) Representative images of induction of apoptosis in breast cancer cells determined by Flow cytometry analysis after 24 h ISL intervention. (D) Chamber invasion assay analysis of the effect of siRNA PTEN transfection on breast cancer motile ability with ISL treatment for 24 h. (E) Percentages of apoptotic cells after ISL treatment with siRNA PTEN intervention. (F) Percentages of the number of cells invading in the ISL treatment with siRNA PTEN interference. (G) Western blot analysis of PTEN function on Bcl-2 down-regulation and Bax up-regulation by ISL. The full-length blots were presented in the Supplementary Fig. 10. Data represent the mean ± s.d. *P < 0.05, **P < 0.01.