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. 2017 Aug 21;8:309. doi: 10.1038/s41467-017-00355-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Brassinosteroid signaling regulates root responses to elevated ambient temperature. a Primary root length of 15-day-old wild-type (WT) or brassinosteroid-related mutant plants grown at 21 or 26 °C (mean ± s.d., n = 25). b Ratio of primary root length from plants grown at 26/21 °C as in a (mean ± s.d., n = 3). The asterisks indicate a statistically significant difference (Kruskal–Wallis; *P < 0.01; **P < 0.001; ***P < 0.0001). c Western blot analyses monitoring the phosphorylation state of BES1 in wild-type plants grown at 21 or 26 °C using anti-BES1 antibodies. A root protein extract from plants treated with brassinolide (BL) is used as a control for BES1 dephosphorylation. A representative blot is shown. d Ratio of phosphorylated BES1 (P-BES1) over BES1 levels (mean ± s.d., n = 4). The asterisk indicates a statistically significant difference (Mann–Whitney; *P < 0.01; **P < 0.001; ***P < 0.0001). e mRNA accumulation of the brassinosteroid feedback-regulated CPD and STE1/DWF7 genes in roots from wild-type plants grown at 21 or 26 °C (mean ± s.d., n = 3). The asterisk indicates a statistically significant difference (Mann–Whitney, P < 0.01). f mRNA accumulation of the BAS1/CYP72B1 and SOB7/CYP72C1 brassinosteroid metabolism genes in roots from wild-type plants grown at 21 or 26 °C (mean ± s.d., n = 3). The asterisk indicates a statistically significant difference (Mann–Whitney, P < 0.01)