Figure 1.

Schematic of the process for bispecific antibody production using the split intein Npu DnaE trans-splicing activity. Three steps were involved, the first was the expression of the fragment A and B in mammalian cells respectively; the second was trans-splicing of the fragment A and B; and the last was purification of the BsAb. Fragment A and B were harvested after cell culture separately, then after primary purification followed by trans-splicing reaction, the two fragments were ligated to form the BsAb.