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. 2017 Aug 21;7:8360. doi: 10.1038/s41598-017-08641-3

Figure 2.

Figure 2

“BAPTS” technology mediated generation of the BsAb (CD3 × HER2). (a) SDS-PAGE (4 – 20%) analysis of the fragments A and B under non-reduced and reduced conditions. All lanes were excised from two gels (Supplementary Figure 1a, lanes 4 and 11; Supplementary Figure 1b, lanes 6 and 13) run under the same condition. (b,c) Catalytic trans-splicing reaction between fragments A and B at 37°C in the absence or presence of various concentrations of reducing agent DTT for 2 hours. The expected trans-spliced product BsAb started to appear at 0.01 mM DTT and the reaction reached plateau at 0.5 mM DTT. (d) SDS-PAGE (4 – 20%) of the reaction between fragments A and B performed at 37°C in the presence of 0.5 mM DTT. About 90% of raw material was converted to BsAb within 25 min after the reaction started.