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. 2017 Aug 21;7:8976. doi: 10.1038/s41598-017-02759-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chronic ethanol feeding induced hepatic apoptosis and increased hepatic HODE levels. WT male mice were fed with control (PF) or alcohol (AF) liquid diet for 8 weeks. (A) TUNEL staining of liver sections. (B) Immunoblot of proteins involved in apoptosis. Protein levels were quantitated by NIH image J. (C) Hepatic HODE and HETE levels measured by LC-MS. All values are denoted as means ± SD (n = 3 in B, n = 6 in C). *P < 0.05, **P < 0.01. HETE: hydroxyeicosatetraenoic acids, HODE: hydroxyoctadecadienoic acid, PUFA: polyunsaturated fatty acids.