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. 2017 Aug 22;7:9160. doi: 10.1038/s41598-017-08827-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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IFN signalling and the antiviral response result from the DME-induced dsRNA formation. (a) Abundance and expression of IFN β in 293T-GFP and 293T-DMEΔ cells. (b) Northern blot analysis for the L1 elements in 293T-GFP and 293T-DMEΔ cells. (c) Abundance and expression of IFN β in the HEK-293T cells 36 h after poly (I:C) treatment. (d), (e) qRT-PCR analysis of the ISGs (d), and cell cycle components and HSPs (e) in the HEK-293T cells 36 h after the poly (I:C) treatment. All blot and gel images were cropped for better display. Error bars represent mean ± S.D. of three independent experiments. *p < 0.05; paired sample t-test.