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. 2017 Aug 22;7:9166. doi: 10.1038/s41598-017-09558-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of hnRNPA2/B1 on Sp1-mediated lung cancer migration. (A,B) Cell mobility was assayed with a wound healing assay (a) and transwell assay (b) in hnRNPA2/B1 knockdown (A) or myc-hnRNPA2/B1-overexpressed cells (B). After three independent experiments, the migratory area and cell number were quantified, and statistical analysis was done with a t-test, *p < 0.05; ***p < 0.005. (C) The protein levels and cell mobility were assayed on the hnRNPA2/B1-silenced cells with or without GFP-Sp1 expression by Western blotting with antibodies against the indicated proteins (a) and by transwell assay (b). After three independent experiments were finished, the cell number on the chamber was quantified and the statistically analysis was performed with t-test, ***p < 0.005.