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. 2017 Aug 22;7:9157. doi: 10.1038/s41598-017-08837-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of serine 110 or 113 point mutations in the PKC recognition motif on mRNA export. (A) RNA FISH analysis shows differential distribution of nuclear and cytoplasmic RNA in HeLa cells treated with siRNA specific for SUN1 and transfected with GFP-SUN1^R expression vectors carrying the indicated mutations. Cells not expressing GFP fusion proteins represent SUN1 depleted cells. Size bar, 10 µm. (B) The nuclear/cytoplasmic (N/C) ratio of the poly(A)+RNA distribution in the various strains as determined by measuring the fluorescence intensity. The percentages of cells in the indicated N/C intensity ranges are shown. The results from one typical experiment are shown. The graph on the right shows the mean values with standard deviations (P value, ***< 0.001). (C) The graph on the right shows the mean values with standard deviations (P value, ***<0.001). (D) Effect of the S to D mutations in GST-SUN1-NT on the interaction with NXF1 and NUP153. GST and GST fusion proteins were used to pull down NXF1 and Nup153 from HeLa cell lysates. The PonceauS stained gel part shows the GST fusion proteins.