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. 2017 Aug 22;7:9073. doi: 10.1038/s41598-017-08290-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DNA immobilization on the probe. (a) Process for immobilizing the DNA on the surface of the probe. (b) Fluorescence imaging of the ssDNAs ([(dA)₅₀-(dC)₅₀]_m) on the surface of the Si probe when the ssDNAs were bound with glutaraldehyde by peptide coupling. (c) Fluorescence image of the ssDNAs ([(dA)₅₀-(dC)₅₀]_m) on the surface of the Si probe when the ssDNAs were not bound with glutaraldehyde by peptide coupling.