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. 2017 Aug 22;7:9040. doi: 10.1038/s41598-017-08855-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Average relative heart rate in different groups (n = 5–6). The heart rate was recorded in each animal by ECG. The average of every 10 s was calculated. The average relative heart rate in each group is shown with different colours. Intravenous pituitrin was injected to induce bradycardia 5 min after recording (indicated as “Modelling”, dashed line). EA was applied at PC 6 at 3 min after modelling (shadow area).