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. 2017 Aug 22;7:9153. doi: 10.1038/s41598-017-09655-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of BHX on cell viability of MDA-MB-231 cells. (a) MCF-10A and MDA-MB-231 cells were treated with different concentrations of BHX for 72 h. (b) Cell viability was decreased in MDA-MB-231 cells at the indicated time points. (c) BHX suppresses colony formation of MDA-MB-231 cells. Results are expressed as means ± S.D. from three independent experiments. ^# p < 0.05 and ^## p < 0.01. (d) Morphological changes of MDA-MB-231 cells 24 h after incubation with BHX (200×). Black arrows indicate cell shrinkage and cell fragmentation.