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. 2017 Aug 22;7:9041. doi: 10.1038/s41598-017-09002-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transient depletion of r-proteins via Dox-inducible shRNA identifies Rps19 as an inducer of cytoprotection against CPT and MTX. (a) The indicated shRNAs were either induced with Dox for 48 h or not induced, cells were treated with 0 or 0.4 μM CPT for 24 h and the surviving viable cells were stained with crystal violet after a 5-day recovery in Dox-free medium. (b) Rps19 shRNA was induced for 48 h with Dox or not induced, cells were treated with the indicated concentrations of CPT for 24 h or MTX for 48 h and stained as above. (c) Dox induction of Rps19 shRNA confers protection against CPT and MTX, but not mitoxantrone. Cells were induced for 48 h with Dox or not induced and then treated with various concentrations of CPT and mitoxantrone for 24 h or with MTX for 48 h. Results were analyzed as in Fig. 2. (d) p53 is necessary for cytoprotective effects of Rps19 depletion. 3T3 cells and its p53-null derivative line MA1.2 were transfected with Dox-inducible Rps19 shRNA and analyzed as in Fig. 3b.