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. 2017 Aug 22;7:9075. doi: 10.1038/s41598-017-08699-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry of CXCR4 expression on CD4⁺ and CD8⁺ T-cells. (A) Scatter plots of flow cytometry. Gating on the CD3⁺ T-cells, the frequencies of CD4⁺ and CD8⁺ T-cells were calculated. Gating on the CD4⁺ T-cells and the CD8⁺ T-cells allowed us to calculate the frequencies of CD4⁺CXCR4⁺ T-cells and CD8⁺CXCR4⁺ T-cells, respectively. (B) Representative histograms of CXCR4 expression on CD4⁺ and CD8⁺ T-cells for three groups. SAA: severe aplastic anaemia; NSAA: non-severe aplastic anaemia; HC: healthy control.