Table 2.
BRCA1 nuclear staining patterns in germline BRCA1 and BRCA2 mutant tumors
| Germline statusa | Total n | TUMORb | NORMALb | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Positive | Het Positive | Negative | Positive | Het Positive | Negative | ||||||||
| n | % | n | % | n | % | n | % | n | % | n | % | ||
| BRCA1, LOHpos | 25 | 0 | 0 | 6 | 24 | 19 | 76 | 10 | 40 | 5 | 20 | 10 | 40 |
| BRCA1, LOHneg | 5 | 1 | 20 | 3 | 60 | 1 | 20 | 3 | 60 | 1 | 20 | 1 | 20 |
| BRCA2 | 13 | 10 | 77 | 2 | 16 | 1 | 7 | 5 | 38 | 5 | 38 | 3 | 23 |
aLOHpos refers to presence of locus-specific LOH LOHneg refers to absence of locus-specific LOH
bFor all samples, immunohistochemical staining for BRCA1 was graded from 0 to 3+ in three cores in both tumor nuclei and normal tissue nuclei with mutation status blinded. The maximum score is shown for nuclear staining. Positive nuclear staining was defined as 100% of nuclei with at least 1+ staining in all three cores. Heterogeneous positive staining was defined as >25% of nuclei with at least 1+ staining in at least one of three cores