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. 2017 Aug 22;7:9042. doi: 10.1038/s41598-017-07995-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Normalized Illumina read mapping to the SMRT assembly of Forc016 confirms horizontal transfer of chr^RC in a Fo47 background. (A) Reads mapped more abundantly to the transferred chr^RC sequence than the rest of the assembly. (B) HCT strains #2, #4, #9 showed a relative (compared to total # mapped reads) depth of coverage of ~4 on chr^RC, whereas the relative coverage of HCT#8 sequences was ~8 along the entire chromosome. This indicates a chromosomal duplication, in accordance with the ~5Mb-sized band in the CHEF gel in Fig. 5.