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. 2017 Aug 22;7:9042. doi: 10.1038/s41598-017-07995-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chr^RC and the two smaller accessory chromosomes of Forc016 are conditionally dispensable. Electrophoretic karyotyping shows complete absence of chr^RC (marked with a red asterisk in lane 6) in all five hygromycin sensitive strains (arrowhead 1, lanes 1–5). Additionally, chromosome loss strain #2 displays absence of the two smallest chromosomes (arrowheads 2). The left and right lanes show the karyotypes of S. pombe and S. cerevisiae, respectively, applied as markers. This image is cropped, the original gel photograph can be found in Supplementary Fig. S13.