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. 2017 Aug 22;7:9148. doi: 10.1038/s41598-017-09542-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduced physiological performance and cellular tolerance of gene silenced maize plants to drought stress. Five plants at 14 dpi were subjected to drought stress and stress response was measured on fifth day. Photosynthesis rate (a), Efficiency of PSII in light adapted leaves (b), and Instantaneous water use efficiency (WUEi) (c) were measured using portable photosynthesis system. Cell membrane stability (d) and Osmolality (e) were measured from the leaf tissue. Cell membrane damage was calculated as per cent injury. BMV-inoculated plants were used as reference for statistical analysis. The values are means ± SE (n = 5). * and ** indicate significant difference from BMV-inoculated plants at P ≤ 0.05 and P ≤ 0.01, respectively. WT, non-inoculated; BMV, vector inoculated; all others with BMV:: followed by gene name are respective gene-silenced plants.