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. 2017 Aug 22;7:9148. doi: 10.1038/s41598-017-09542-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Improved osmotic stress tolerance of AtGBF3 and EcGBF3 transgenic Arabidopsis plants. A week old hygromycin resistant T₃ transgenic seedlings along with Col-0 and Atgbf3 mutant seedlings were transferred on to medium (1/2 MS, 0.5% sucrose and 0.1% phytagel) containing 100 mM mannitol and growth performance was measured after 10 days. Root length (a) and Biomass (b) were measured using plants carefully uprooted from the media and blotted on paper towel to remove any media. The values are means ± SE (n = 10). t test was used to show the statistical significance of the differences in the means of Atgbf3 mutant or transgenic plants from Col-0 plants. * and ** indicate significant difference from Col-0 plants at P ≤ 0.05 and P ≤ 0.01, respectively. Atgbf3, AtGBF3 mutant; AtGBF3-2, -3 and -4, transgenic lines overexpressing AtGBF3; mAtGBF3-1, 3 and -5, transgenic lines overexpressing AtGBF3 in the Atgbf3 mutant background; EcGBF3-1, 2 and 3, transgenic lines overexpressing EcGBF3; mEcGBF3-2, 3 and 4, transgenic lines overexpressing EcGBF3 in the Atgbf3 mutant background.