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. 2017 Aug 22;7:9089. doi: 10.1038/s41598-017-09664-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tibetan chickens and their tibiae grow slowly compared to AA chickens. (a) Feeding location (experimental site) of the TBCs (2,986 meters above sea level; Tibet, China) and AA chickens (50 meters above sea level; Wuhan, China). (b) Standard for measurement of the T. Length (tibia length) and T. G. P. Width (tibial growth plate width). (c,d,f,g) The body weights and tibia weights of the chickens and the lengths and growth plate widths of the tibiae were recorded and compared between the TBCs and AACs. (e) The tibial weight index was determined as the tibial weight per corresponding body weight of the chicken (T. Weight/body weight, mg/g) between the TBCs and AACs. (h) The tibial growth plate index was determined as the tibial growth plate width per tibia length (T. growth plate width/T. length, µm/mm) between the TBCs and AACs. Student’s t-test, ^*** p < 0.001, n = 16; Error bars indicate SD. AAC, Arbor Acres chicken; TBC, Tibetan chicken.