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. 2017 Aug 22;7:9038. doi: 10.1038/s41598-017-09383-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of supplementation with various concentrations of an osmotic stabilizer on fungal growth. Colony morphologies on PDAmb supplemented with corresponding concentrations of sorbitol are shown after 10 d of cultivation. Strains are wild-type EP155/2, UEP1, TdSLT2-69, and the sectored TdSLT2-69 progeny, TdSLT2-69-S1. Note that colonies with the same CpSlt2 mutant background, such as TdSLT2-69 and TdSLT2-69-S1, showed more severe growth defects when cultivated on plates containing 1.0 M sorbitol. On the other hand, wild type and its isogenic hypovirulent UEP1 strains cultivated in the presence and absence of 1.0 M sorbitol were not significantly different.