Figure 6.

(A) X-ray diffractograms of the film. The peaks represented in green (F1) are for the film prepared using increased concentration of PHB, Nm and glycerol blend (2:1:1) with high intensity peaks, indicating the crystallinity of the film. Peaks shown in black (F2) correspond to the film prepared using equal proportion of the components Nm: PHB: glycerol (1:1:1) and displayed a similar diffraction pattern as the peak obtained with the increased concentration of PHB. Peaks represented in red (F3) are the film prepared using increased melanin: PHB: glycerol (2:1:1) which displayed a broader peak in the diffraction pattern of 20.2° to 26.8°. (B,C) Figure shows the AFM images of the samples scanned in an area of 4μm². AFM topographical images of melanin nanocomposite film prepared with equal proportion of PHB, Nm and glycerol blend (1:1:1). The topographical scale of the images in 3D (B) and 2D (C) are shown in 50 nm scales. The roughness of the film was found to be 12.4 nm.