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. 2017 Aug 22;7:9099. doi: 10.1038/s41598-017-09329-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow chart diagram illustrating the potential use of the dual MS-AFST in clinical practice. A scenario of prior echinocandin exposure is shown, where MS-AFST results for a C. glabrata clinical isolate as susceptible or resistant to anidulafungin (AND) and/or fluconazole (FLC) may be useful to drive the appropriate administration of antifungal therapy. For AND, valid MS-AFST results can be available after 3 h or, in cases of isolates with FKS2 HS1 mutations, 6–12 h of antifungal exposure in vitro.