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. 2017 Aug 22;7:9056. doi: 10.1038/s41598-017-09021-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) CT histograms of lung ROI from a representative irradiated rat over time from week 2 to 24 post-RT. Histograms represents the portion of voxels within the lung ROI at a certain density (HU). (b) CT histograms for all groups at week 24 post-RT. (c) Cumulative histograms at week 24 post-RT. Dotted line at the 75% intercept line to maximize separation between groups. (d) HU value of the 75% intercept from the cumulative histogram for each group. Bar represents the mean with 95% CI and each point is a rat. There is a significant difference between the Control and all RT groups. Dashed line was set at the mean of the RT group. (e) Percent of rats exhibiting a response to treatment. Rats falling under the mean of the RT groups (dashed line from (d)) are considered “responders” (closer to Controls) and rats above the RT mean are considered “non-responders”.