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. 2017 Aug 22;7:9162. doi: 10.1038/s41598-017-09149-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of high calorie environment towards visceral adiposity and adipocyte histology in genetically different rat strains. After the completion of feeding experiment, rats (WNIN, F-344 and SD) were sacrificed and assessed visceral adiposity and adipocyte histology. (A) Dissection weights of white adipose tissue (WAT). (B) Adiposity index was calculated to measure central adiposity. (C) Hematoxylin-Eosin (H&E) staining was performed to determine the morphology of retro adipose tissue. (D & E) Mean adipocyte area and adipocyte number was calculated in retro adipose tissue. (F–H) Fraction of distribution of adipocytes in retroadipose tissue. Diets: control; HF, high fat; HS, high sucrose; HFS, high fat sucrose. *P < 0.05; **P < 0.01; ***P < 0.001 statistically significance compared to their respective controls s. Groups were compared using one way ANOVA.