Table 1.
Expression of pro-inflammatory cytokines in visceral adipose tissue.
| Conc. (pg/mg) | WNIN | F-344 | SD | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Control | HF | HFS | Control | HF | HFS | Control | HF | HFS | |
| IL-6 | 1.91 ± 0.25 | 4.42 ± 0.53 ** | 5.05 ± 0.308 ** | 2.22 ± 0.21 | 2.54 ± 0.205 | 2.06 ± 0.244 | 3.15 ± 0.135 | 3.45 ± 0.305 | 3.51 ± 0.117 |
| TNF-α | 1.4 ± 0.125 | 6.05 ± 0.612 ** | 5.75 ± 0.663 ** | 0.54 ± 0.058 | 0.773 ± 0.124 | 0.68 ± 0.147 | 0.863 ± 0.159 | 0.95 ± 0.121 | 1.11 ± 0.091 |
| MIP-1α | 0.91 ± 0.288 | 3.59 ± 0.655 ** | 4.25 ± 0.345 ** | 0.953 ± 0.06 | 1.19 ± 0.055 | 1.23 ± 0.096 | 1.13 ± 0.064 | 1.3 ± 0.181 | 1.21 ± 0.239 |
| MCP-1 | 7.26 ± 0.796 | 13.8 ± 0.47 ** | 15.6 ± 1.36 ** | 5.14 ± 0.591 | 6.21 ± 0.325 | 5.48 ± 0.267 | 6.94 ± 0.288 | 7.93 ± 0.085 | 7.44 ± 0.5 |
Retroperitoneal fat was used for the preparation of total tissue lysate and estimated the protein levels of pro-inflammatory cytokines such as interleukin 6, IL-6; tumor necrosis factor alpha, TNFα; macrophage inflammatory protein 1 alpha, MIP-1α; monocyte chemotactic protein 1, MCP1 among WNIN, F-344 and SD rats under high calorie environment. Data was presented as mean ± SEM (n = 6 per group). Diets: control; HF, high fat; HS, high sucrose; HFS, high fat sucrose. *P < 0.05; **P < 0.01; ***P < 0.001 statistically significance compared to their respective controls. Groups were compared using one way ANOVA.