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. 2017 Aug 22;7:9105. doi: 10.1038/s41598-017-08770-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The lipid raft association of calflagin is affected differently by methyl-β-cyclodextrin (MBCD) and amphotericin B (Amp B) treatment of bloodstream and procyclic cells. Lipid raft extracts were prepared from T. brucei bloodstream and procyclic cells with or without pretreatment with MBCD or Amp B. Extracts were run on Optiprep gradients and gradient fractions were analyzed by western blotting using antibodies specific for calflagin (lipid raft) and PFR2 and mt-hsp70 (non-raft controls). I = input material loaded on the gradient.