Table 1.

Sterol composition of procyclic cell bodies and flagella, normalized to the protein concentration in the sample.

Peak	Sterol Species	Cell Body (pmol/mg protein) (relative abundance %)	Flagellum (pmol/mg protein) (relative abundance %)	P value**	Flagellum: Cell Body Ratio***
1	Cholesterol	1.45 ± 0.09* (47.6)	6.02 ± 0.34 (52.1)	0.0008	4.2
2	Zymosterol	0.14 ± 0.001 (4.6)	0.19 ± 0.006 (1.7)	0.13	1.4
3	Cholesta-5,7,24-trienol	0.72 ± 0.01 (23.5)	1.57 ± 0.03 (10.2)	0.006	2.2
4	Ergosta-5,7,25(27)-trienol	0.52 ± 0.05 (17.1)	2.69 ± 0.15 (22.4)	0.009	5.2
5	Lanosterol	0.22 ± 0.01 (7.3)	0.51 ± 0.05 (13.6)	0.013	2.3
	Total	3.04 ± 0.16 (100)	11.54 ± 0.58 (100)		3.8

*Mean ± SEM. **Student’s T-test. Paired comparison of sterol levels normalized by protein concentration from Cell Body and flagellum using two biological replicates and three technical replicates. ***Ratios when normalized to procyclin instead of protein concentration are (top to bottom) 6.8, 1.8, 2.9, 4.5, 4.0, 5.0.