Table 1.
Peak | Sterol Species | Cell Body (pmol/mg protein) (relative abundance %) | Flagellum (pmol/mg protein) (relative abundance %) | P value** | Flagellum: Cell Body Ratio*** |
---|---|---|---|---|---|
1 | Cholesterol | 1.45 ± 0.09* (47.6) | 6.02 ± 0.34 (52.1) | 0.0008 | 4.2 |
2 | Zymosterol | 0.14 ± 0.001 (4.6) | 0.19 ± 0.006 (1.7) | 0.13 | 1.4 |
3 | Cholesta-5,7,24-trienol | 0.72 ± 0.01 (23.5) | 1.57 ± 0.03 (10.2) | 0.006 | 2.2 |
4 | Ergosta-5,7,25(27)-trienol | 0.52 ± 0.05 (17.1) | 2.69 ± 0.15 (22.4) | 0.009 | 5.2 |
5 | Lanosterol | 0.22 ± 0.01 (7.3) | 0.51 ± 0.05 (13.6) | 0.013 | 2.3 |
Total | 3.04 ± 0.16 (100) | 11.54 ± 0.58 (100) | 3.8 |
*Mean ± SEM. **Student’s T-test. Paired comparison of sterol levels normalized by protein concentration from Cell Body and flagellum using two biological replicates and three technical replicates. ***Ratios when normalized to procyclin instead of protein concentration are (top to bottom) 6.8, 1.8, 2.9, 4.5, 4.0, 5.0.