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. 2017 Aug 22;7:9117. doi: 10.1038/s41598-017-08810-4

Figure 2.

Figure 2

The Eng1 Cre, HoxA2-Cre, Egr2 Cre, HoxB1 Cre, and HoxA4-Cre drivers capture broad rhombomeric domains in the embryo and adult. The five rhombomere-specific-Cre lines were crossed with Ai9 mice to express tdTomato in a Cre-specific manner. In column 1, rhombomere-Cre; Ai9 E10.5 whole embryos were cleared using hydrogel cross-linking followed by lipid extraction by SDS treatment. In columns 2–4, DAPI and tdTomato fluorescence of sagittal brainstem cryosections (30 μm) from adult rhombomere-Cre; Ai9 animals are shown. Embryo and adult expression can be seen in the Eng1 Cre (A–D), HoxA2-Cre (E–H), Egr2 Cre (I–L), HoxB1 Cre (M–P), and HoxA4-Cre (Q–T) domains.