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. 2017 Aug 22;7:9117. doi: 10.1038/s41598-017-08810-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CNO-hM4D perturbation of rhombomeric domains in the adult animal results in significant reductions in body temperature. Temperature was taken at the start of respiratory assays (Pre-CNO Beginning), immediately before CNO injection (Pre-CNO Middle), at the end of respiratory assays (Post-CNO End), and 30 minutes at room temperature after the end of the assay (Post-CNO 30 min). Statistical significance was determined using a linear mixed-effects regression model with animal type (experimental or control) as a fixed effect. *p < 0.05, **p < 0.01, ***p < 0.001.