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. 2017 Aug 22;7:9126. doi: 10.1038/s41598-017-09070-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Qualitative comparison of ROX SE perfusion and India ink-gelatin casting techniques. Naïve wild-type mice underwent ROX SE perfusion followed by 3% India ink-gelatin casting. Images were then obtained of the mouse brains to compare the India ink-gelatin casting method (A,C,E,G) and ROX SE perfusion method (B,D,F,H). Images obtained using the ROX SE perfusion technique did not have problems of poor visualization of overlaying vessels (E, indicated by arrowheads) or poor perfusion (G, indicated by arrowheads). Scale bar: 100 μm.