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. 2017 Aug 22;7:9126. doi: 10.1038/s41598-017-09070-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of immunohistochemical staining for microvessel thrombosis between ROX SE perfusion and India ink-gelatin casted sections. Anti-fibrinogen immunohistochemistry in the ROX SE perfused brain sections (C,D) revealed increased microvessel thrombosis in mice that underwent SAH compared to sham surgery. No difference was seen in the India ink-gelatin casted brain sections due to obscuration of vessel lumen by India ink (A,B). Scale bar: 200 μm.