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. 2017 Aug 22;7:9024. doi: 10.1038/s41598-017-07886-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genome structure of the LpBGNL and E. festucae ß-1,6-glucanase genes and genetic linkage analysis for LpBGNL. (a) Alignment of genome sequences from perennial ryegrass and Epichloë species. The light green-filled and striped boxes show the location of LpBGNL and the plant genome-related sequence, respectively. The black-filled box represents the BAC vector. The purple-filled and empty boxes show the location of the ß-1,6-glucanase gene and flanking gene, respectively. The transcription direction of the genes is indicated with the arrow. Corresponding gene sequences are connected with black dashed lines. (b) Alignment of coding regions of the LpBGNL and Epichloë ß-1,6-glucanase genes. The light green and purple lines represent non-gene coding region of perennial ryegrass and Epichloë species. The grey line and pink breaking line show the location of the intron and aryl-phospho-beta-D-glucosidase domain, respectively. (c) Genetic linkage map of perennial ryegrass LG5 with the LpBGNL-related locus. The LpBGNL-related marker locus is indicated with the red arrow. Genetic distance (cM) is shown on the right side of the genetic markers.