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. 2017 Aug 22;7:9024. doi: 10.1038/s41598-017-07886-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic tree of LpBGNL orthologues and fungal ß-1,6-glucanase genes. The phylogram was generated based on amino acid sequence of the aryl-phospho-beta-D-glucosidase domain, and sequences from angiosperm and fungi species are indicated with green and purple, respectively. Asterisk (*) denotes species from which gene products have been confirmed to have the ß-1,6-glucanase activity. For cocksfoot, three contigs (haplotypes 1–3) were generated, and amino acid sequences from those contigs were used. For Dactylis marina, the contig without a putative premature stop codon was used. Strain and sequence contig (scaffold) identifiers of the Genome Project at the University of Kentucky website are shown in brackets. For the other fungi sequences, NCBI UI is shown in bracket. The clade including the sequences from Trichoderma and Hypocreaas species were selected as an outer group to obtain a root of the phylogenic tree. A figure legend generated with the MEGA7 software can be found in Supplementary Note S1.