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. 2017 Aug 22;7:9119. doi: 10.1038/s41598-017-08672-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic tree of OMTs. Phylogenetic tree was constructed based on the deduced amino acid sequences for the D. scandens OMTs (bold letters) and other plant OMTs. Abbreviations and GenBank accession numbers for the sequences used are as follows: EcOMT, putative Eschscholzia californica OMT (ACO90220.1); CjCoOMT, Coptis japonica columbamine OMT (Q8H9A8.1); TtOMT, Thalictrum tuberosum catechol OMT (AAD29845.1); TtCaOMT, T. tuberosum catechol OMT (AAD29843.1); PsCaOMT, P. somniferum catechol OMT (AAQ01670.1); PsN7OMT, P. somniferum norreticuline 7OMT (ACN88562.1); Ps6OMT, P. somniferum norcoclaurine 6OMT (AAP45315.1); Ps4′OMT2, P. somniferum 3′-hydroxy-N-methylcoclaurine 4′OMT2 (AAP45314.1); Ps4′OMT1, P. somniferum 3′-hydroxy-N-methylcoclaurine 4′OMT1 (AAP45314.1); Cc4′OMT, Coptis chinensis 3′-hydroxy-N-methylcoclaurine 4′OMT (ABY75613.1); Cj4′OMT, C. japonica 3′-hydroxy-N-methylcoclaurine 4′OMT (Q9LEL5.1); Tf4′OMT, T. flavum 3′-hydroxy-N-methylcoclaurine 4′OMT (AAU20768.1); Cj6OMT, C. japonica norcoclaurine 6OMT (Q9LEL6.1); Tf6OMT, T. flavum norcoclaurine 6OMT (AAU20765.1); VvReOMT, Vitis vinifera resveratrol OMT (CAQ76879.1); PtFlOMT, Populus trichocarpa flavonoid OMT predicted protein (XP_002312933.1); CjSOMT, C. japonica scoulerine 9OMT (Q39522.1); TfSOMT, T. flavum scoulerine 9OMT(AAU20770.1); PaCafOMT, Picea abies caffeate OMT (CAI30878.1); CaCafOMT, Capsicum annuum caffeate OMT (AAG43822.1); PsOMT1, P. somniferum SOMT1 (JN185323); PsOMT2, P. somniferum SOMT2 (JN185324); PsOMT3, P. somniferum SOMT3 (JN185325); ObEuOMT, Ocimum basilicum eugenol OMT (AAL30424.1); MpFlOMT, Mentha X piperita flavonoid 8OMT (AAR09600.1); ObCafOMT, O. basilicum caffeate OMT (AAD38189.1); CbEuOMT, Clarkia breweri (iso)eugenol OMT (AAC01533.1); CbCafOMT, C. breweri caffeate OMT (O23760.1); and AmCafOMT, Ammi majus caffeate OMT (AAR24095.1).