Table 1.
Models of In Vivo Corneal Angiogenesis.
| Experimental Model | Investigation focus | Intravital imaging modality* | Vascular network architecture | Single-cell resolution | Protein expression | Serial imaging | Blood velocity | sO2 | Distinguish depth of vascular bed | Citation |
|---|---|---|---|---|---|---|---|---|---|---|
| Micropocket (tumor) | Tumor vascularization | Bright field microscopy | ✓ | ✓ | 36, 37 | |||||
| Micropocket (growth factor) | Evaluation of angiogenic growth factors in multiple mouse genotypes | Slit lamp microscopy | ✓ | ✓ | 38 | |||||
| Micropocket (pancreatic islet) | Pancreatic islet vascularization; β-cell function and rate of death | Confocal microscopy | ✓ | ✓ | ✓ | 39 | ||||
| Micropocket (adipose-derived stem cells (ASCs)) | ASCs in HLA scaffolds; corneal regeneration | Bright field microscopy | ✓ | 40 | ||||||
| Micropocket (hydrogel scaffold) | Live imaging of biomaterials (compatibility, vascularization) | Confocal microscopy | ✓ | ✓ | ✓ | ✓ | 41 | |||
| Topical treatment (anti-VEGF) | Anti-angiogenic therapy (human trial) | Bright field microscopy | ✓ | ✓ | 42 | |||||
| Suture | Lymph-angiogenesis | Confocal microscopy | ✓ | ✓ | ✓ | ✓ | 11 | |||
| Chemical burn | Detect vessels in cornea with PAM | Photoacoustic microscopy | ✓ | ✓ | 43 | |||||
| Chemical burn | EC Tie2 expression | Photoacoustic and confocal microscopy | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | Present study |
*Used at the highest resolution.