FIG. 7.
Cell death in OHSCs is correlated with OxDs. (A–C) Fluorescence images of OHSCs stained by PI after different treatments. (A) Live control is OHSC without any treatment (assigned 0% cell death). Dead control is OHSC treated with methanol (assigned 100% cell death). The calculation of cell death percentage is described in the Experimental Methods section in Supplementary Data. (B, C) Differential mortality of pyramidal cells in areas CA1 and CA3 is found after the treatment with OGD-RP and 10 μM MD +20 μM MnSODm. Data are represented as mean ± SEM (n = 10 OHSCs) and analyzed with one-way ANOVA test. See Figure 1 legend for indicators of statistical significance. (D) Correlation tests were run on data acquired in treatments included in (B, C). Pearson's correlation coefficient, R, is notated at each subplot and highlighted in red when p < 0.05, indicating that the correlation is significant. Cell death percentage is positively correlated with OxDP and OxDG. PI, propidium iodide.