Figure 6.

Effect of EtOH-Ext, Hex-F, Lupeol, and the reference drug Amph B (2 μg/mL) on the infected macrophages (a) and the survival index of macrophage-internalized amastigotes (b) after 48 h of exposure. Murine peritoneal macrophages were infected with promastigote forms of L. amazonensis and then treated with concentrations related to respective IC₅₀, 1/2 IC₅₀, and 1/4 IC₅₀ against promastigotes. Results represent the means ± SEM of three experiments performed in triplicate. ^∗∗∗P < 0.001 versus control.