Skip to main content
. 2017 Aug 22;12(8):e0183346. doi: 10.1371/journal.pone.0183346

Fig 2. SDS-PAGE analysis of the PA, LF, EF and HtrA proteins (Coomassie stained).

Fig 2

Proteins were purified from BH500 strains containing pYS5, pSJ115, pSJ136EFOS and pUTE29-htrA plasmids, respectively. The strains were grown in FA medium with 20 μg/ml of kanamycin (or 10 μg/ml tetracycline for pUTE29-htrA) at 37°C for 14 h. Protein purification was performed following procedures described in the Materials and Methods section. Mr—PageRuler unstained protein ladder mix for size determination (e.g., 85 kDa).