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. 2017 Aug 22;12(8):e0183346. doi: 10.1371/journal.pone.0183346

Table 1. Bacterial strains used in this study.

B. anthracis strains Relevant characteristic(s) Source or reference
Ames 35 B. anthracis (pXO1+, pXO2-), from Ames strain by removal of pXO2. [7]
Ames 35 ΔAtxA Ames 35 with atxA gene deleted [12]
BH460 pXO1-, pXO2-, Spo0A-, NprB-, TasA-, Cam-, InhA1-, InhA2-, MmpZ-; total of five loxP sites in chromosome, six proteases inactivated [9]
BH470 BH460 with cysP1 knockout containing one FRT site This work
BH480 BH470 with vpR knockout containing second FRT site in chromosome This work
BH490 BH480 with nprC knockout containing third FRT site in chromosome This work
BH490A BH490 with the first atxA located upstream of the third FRT site This work
BH490A2 BH490A with the second atxA located upstream of the first atxA copy; sixth loxP site is located between two atxA This work
BH490A3 BH490A2 with the third atxA located downstream of the sixth loxP site and between two originally inserted atxA; contains one PSL site in chromosome This work
BH500 BH490 with s41 knockout containing one PSL site in chromosome This work