Fig. 3.

GA promoted the expression level of SIRT1 and suppressed the NF-κB signaling pathway. C57BL/6 mice and HK-2 cells were treated with LPS with or without GA. Western blot was performed to determine the expression levels of SIRT1 in renal tissues (a, b) and HK-2 cells (e, f). c The activity of NF-κB in renal tissues was measured by ELISA. d The protein levels of p-IκBα and IκBα in renal tissues was examined by western blot. n = 10, *P < 0.05. g, h The levels of iNOS, p-IκBα and IκBα in treated HK-2 cells were detected by western blot. n = 3, *P < 0.05