Table 3.

The new pMMO primers designed based on aligned pmoC, A, and B compared to pmoA189

Primers	Sequence	MT	%GC
PmoC374	5′-AGCARGACGGYACNTGGC-3′	42,9	56
PmoA189	5′-GGNGACTGGGACTTCTGG-3′	40,3	56
PmoA344	5′-ANGTCCAHCCCCAGAAGT-3′	42,9	50

MT melting temperature, %GC GC content in percentage