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. 2017 Aug 1;23(4):412–419. doi: 10.1089/ten.teb.2016.0332

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© Kevin R. Stone et al. 2017; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 3. — Anti-Gal (A) and anti-non-gal IgG antibody response (B) in two representative orthopedic patients implanted with engineered pig patellar tendon for the replacement of ruptured ACL. Patient 1, open columns; Patient 2, closed columns. Anti-Gal antibody activity was determined by ELISA with α-gal linked to bovine serum albumin as solid-phase antigen. Anti-non-gal antibody activity was determined with anti-Gal-depleted sera (adsorption on glutaraldehyde-fixed rabbit red cells), by ELISA with homogenate of fragmented pig tendon as solid-phase antigen. The figure shows antibody binding at serum dilution of 1:640 (based on data presented in Ref.²⁸).