Figure 6.

Contribution of the C-terminal extension to the nucleation activity. (A) Shown is the domain diagram of the Lmod1, Lmod2, and Lmod1-Lmod2 hybrid constructs analyzed here. (B) Given here is the time course of polymerization of 2 μM Mg-ATP-actin (6% pyrene-labeled) in the presence of 25 nM of the constructs depicted in (A) or the actin control (as indicated). Note that in these experiments actin alone (control) polymerized at a rate of 0.6 nM s⁻¹. (C) Given here is the concentration dependence of the polymerization rates, displayed as the mean of three independent experiments ± SE. To see this figure in color, go online.