Table 5. Trophozoite-schizont transition proteome oxidative modifications1.
Proteome 1 | Proteome 2 | Control | ||||
---|---|---|---|---|---|---|
peptides: | 5028 | 4377 | 3043 | |||
aa, mod | # | Fraction | # | Fraction | # | Fraction |
met | 1255 | 1449 | 604 | |||
unmodified | 133 | 0.1060 | 899 | 0.6204 | 48 | 0.0795 |
O | 994 | 0.7920 | 491 | 0.3389 | 522 | 0.8642 |
O2 | 128 | 0.1020 | 109 | 0.0752 | 34 | 0.0563 |
tyr | 2162 | 2282 | 1137 | |||
unmodified | 1990 | 0.9204 | 2240 | 0.9816 | 1121 | 0.9859 |
O | 81 | 0.0375 | 13 | 0.0057 | 14 | 0.0123 |
O2 | 12 | 0.0056 | 7 | 0.0031 | 2 | 0.0018 |
O3 | 13 | 0.0060 | 15 | 0.0066 | 0 | 0.0000 |
NO2 | 60 | 0.0278 | 6 | 0.0026 | 0 | 0.0000 |
NO2OH | 6 | 0.0028 | 1 | 0.0004 | 0 | 0.0000 |
trp | 180 | 295 | 401 | |||
unmodified | 122 | 0.6778 | 254 | 0.8610 | 233 | 0.5810 |
O | 25 | 0.1389 | 25 | 0.0847 | 136 | 0.3392 |
O2 | 17 | 0.0944 | 16 | 0.0542 | 26 | 0.0648 |
O3 | 11 | 0.0611 | 0 | 0.0000 | 6 | 0.0150 |
NO2 | 2 | 0.0111 | 0 | 0.0000 | 0 | 0.0000 |
NO2OH | 7 | 0.0389 | 0 | 0.0000 | 0 | 0.0000 |
cys | 457 | 796 | 149 | |||
unmodified | 20 | 0.0438 | 1 | 0.0013 | 0 | 0.0000 |
CAM2 | 403 | 0.8818 | 784 | 0.9849 | 149 | 1.0000 |
O | 16 | 0.0350 | 0 | 0.0000 | 0 | 0.0000 |
O2 | 8 | 0.0175 | 2 | 0.0025 | 0 | 0.0000 |
O3 | 10 | 0.0219 | 9 | 0.0113 | 0 | 0.0000 |
phe | 2639 | 2659 | 1870 | |||
unmodified | 2513 | 0.9523 | 2611 | 0.9819 | 1846 | 0.9872 |
O | 68 | 0.0258 | 30 | 0.0113 | 21 | 0.0112 |
O2 | 32 | 0.0121 | 16 | 0.0060 | 3 | 0.0016 |
NO2 | 13 | 0.0049 | 1 | 0.0004 | 0 | 0.0000 |
NO2OH | 13 | 0.0049 | 1 | 0.0004 | 0 | 0.0000 |
his | 1922 | 2600 | 1259 | |||
unmodified | 1813 | 0.9433 | 2539 | 0.9765 | 1246 | 0.9897 |
O | 42 | 0.0219 | 26 | 0.0100 | 10 | 0.0079 |
O2 | 41 | 0.0213 | 33 | 0.0127 | 3 | 0.0024 |
NO2 | 16 | 0.0083 | 2 | 0.0008 | 0 | 0.0000 |
NO2OH | 10 | 0.0052 | 0 | 0.0000 | 0 | 0.0000 |
1 All peptides have an observed precursor ion mass less than 5 ppm from the theoretical mass;
2 carboxamidomethyl