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. 2017 Aug 22;12(8):e0182561. doi: 10.1371/journal.pone.0182561

Table 5. Trophozoite-schizont transition proteome oxidative modifications1.

Proteome 1 Proteome 2 Control
peptides: 5028 4377 3043
aa, mod # Fraction # Fraction # Fraction
met 1255 1449 604
unmodified 133 0.1060 899 0.6204 48 0.0795
O 994 0.7920 491 0.3389 522 0.8642
O2 128 0.1020 109 0.0752 34 0.0563
tyr 2162 2282 1137
unmodified 1990 0.9204 2240 0.9816 1121 0.9859
O 81 0.0375 13 0.0057 14 0.0123
O2 12 0.0056 7 0.0031 2 0.0018
O3 13 0.0060 15 0.0066 0 0.0000
NO2 60 0.0278 6 0.0026 0 0.0000
NO2OH 6 0.0028 1 0.0004 0 0.0000
trp 180 295 401
unmodified 122 0.6778 254 0.8610 233 0.5810
O 25 0.1389 25 0.0847 136 0.3392
O2 17 0.0944 16 0.0542 26 0.0648
O3 11 0.0611 0 0.0000 6 0.0150
NO2 2 0.0111 0 0.0000 0 0.0000
NO2OH 7 0.0389 0 0.0000 0 0.0000
cys 457 796 149
unmodified 20 0.0438 1 0.0013 0 0.0000
CAM2 403 0.8818 784 0.9849 149 1.0000
O 16 0.0350 0 0.0000 0 0.0000
O2 8 0.0175 2 0.0025 0 0.0000
O3 10 0.0219 9 0.0113 0 0.0000
phe 2639 2659 1870
unmodified 2513 0.9523 2611 0.9819 1846 0.9872
O 68 0.0258 30 0.0113 21 0.0112
O2 32 0.0121 16 0.0060 3 0.0016
NO2 13 0.0049 1 0.0004 0 0.0000
NO2OH 13 0.0049 1 0.0004 0 0.0000
his 1922 2600 1259
unmodified 1813 0.9433 2539 0.9765 1246 0.9897
O 42 0.0219 26 0.0100 10 0.0079
O2 41 0.0213 33 0.0127 3 0.0024
NO2 16 0.0083 2 0.0008 0 0.0000
NO2OH 10 0.0052 0 0.0000 0 0.0000

1 All peptides have an observed precursor ion mass less than 5 ppm from the theoretical mass;

2 carboxamidomethyl