Skip to main content
. Author manuscript; available in PMC: 2018 Apr 17.
Published in final edited form as: Chem Res Toxicol. 2017 Mar 29;30(4):980–995. doi: 10.1021/acs.chemrestox.6b00389

Figure 6.

Figure 6

HPLC-ESI+-MS/MS analysis of dG-Pt-Lys conjugates in total proteolytic digests of chromosomal DNA recovered from cisplatin-treated cells. HT1080 cells were treated with 100 μM cisplatin for 3 h to induce DNA-protein cross-links. Following extraction of the chromosomal DNA containing covalent DPCs, the cross-linked proteins were subjected to enzymatic hydrolysis to release amino acid-nucleobase conjugates. Synthetic dG-Pt-Lys (A); enzymatic digests of DPC mixtures from HT1080 cells incubated in the absence of cisplatin (B); enzymatic digests of DPC mixtures treated with 100 μM cisplatin (C).