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. Author manuscript; available in PMC: 2018 Apr 17.
Published in final edited form as: Chem Res Toxicol. 2017 Mar 29;30(4):980–995. doi: 10.1021/acs.chemrestox.6b00389

Table 1.

Proteins participating in cross-linking to chromosomal DNA in human fibrosarcoma HT1080 cells treated with cisplatin (100 μM for 3 h).

Swiss-Port ID Identified Proteins % Coverage No. of Unique Peptides No. of Assigned Spectra Primary Cellular Function Protein MW (Da)

P62258 14-3-3 Protein epsilon 24 5 11 Cell Signaling/Motility/Architecture 29175.0
P62736 Actin, aortic smooth muscle 24 8 37 42010.1
P60709 Actin, cytoplasmic 1 (β-actin) 35 7 97 41737.8
Q01518 Adenylyl cyclase-associated protein 1 12 4 9 51855.5
P12814 α-Actinin-1 13 10 19 103061.1
O43707 α-Actinin-4 10 6 11 104857.2
P04083 Annexin A1 37 9 22 38715.9
P07355 Annexin A2 58 15 36 38606.1
P23528 Cofilin-1 45 5 15 18503.2
Q07065 Cytoskeleton-associated protein 4 9 4 7 66022.2
Q16643 Drebrin 14 6 10 71428.6
P14625 Endoplasmin 38 24 80 92471.7
P15311 Ezrin 9 5 9 69414.7
P21333 Filamin-A 3 4 6 280729.4
Q99988 Growth/differentiation factor 15 23 6 12 34154.8
P07900 Heat shock protein HSP 90-α 23 13 45 84663.2
P08238 Heat shock protein HSP 90-β 21 12 75 83267.3
O95373 Importin-7 7 5 15 119519.5
P05787 Keratin, type II cytoskeletal 8 42 18 74 53706.2
P02545 Prelamin-A/C 53 34 93 74140.7
P20700 Lamin-B1 36 16 29 66409.6
Q03252 Lamin-B2 22 13 46 67689.8
Q15185 Prostaglandin E synthase 3 42 6 15 18697.9
P61026 Ras-related protein Rab-10 28 5 9 22542.1
Q13813 Spectrin α chain, brain 37 75 161 284542.7
Q01082 Spectrin β chain, brain 1 27 46 91 274613.4
Q9UH99 SUN domain-containing protein 2 10 4 9 80312.2
P09493 Tropomyosin α-1 chain 14 4 4 32710.0
Q71U36 Tubulin α-1A chain 41 14 53 50135.7
P07437 Tubulin β chain 16 4 20 49670.6
Q13885 Tubulin β-2A 36 11 91 49907.1
P68371 Tubulin β-2C chain 20 6 18 49830.7
Q9BUF5 Tubulin β-6 chain 18 4 6 49857.2
P08670 Vimentin 65 28 990 53652.7

P31946 14-3-3 protein β/α 19 5 10 Cellular Homeostasis/Cell Cycle 28083.1
P62244 40S ribosomal protein S15a 29 4 9 14840.0
P62847 40S ribosomal protein S24 29 4 8 15423.8
P61247 40S ribosomal protein S3a 41 10 23 29945.3
P62753 40S ribosomal protein S6 23 5 11 28681.7
P08865 40S ribosomal protein SA 36 8 15 32854.1
P10809 60 kDa heat shock protein, mitochondrial 23 8 19 61055.7
P11021 78 kDa glucose-regulated protein OS=Homo 23 11 24 72334.7
P84077 ADP-ribosylation factor 1 34 4 8 20697.6
P18085 ADP-ribosylation factor 4 22 4 9 20511.6
P15144 Aminopeptidase N 25 18 40 109542.4
Q9UKV3 Apoptotic chromatin condensation inducer in the nucleus 3 4 7 151887.9
P25705 ATP synthase subunit α, mitochondrial 14 7 10 59752.1
P06576 ATP synthase subunit β, mitochondrial 10 4 7 56560.6
O00571 ATP-dependent RNA helicase DDX3X 9 4 8 73245.8
P27824 Calnexin 23 14 36 67570.2
P27797 Calreticulin 52 18 54 48142.9
Q9UQ88 Cell division protein kinase 11A 14 10 22 90976.0
Q68CQ4 Digestive organ expansion factor homolog 12 7 9 87057.4
P62495 Eukaryotic peptide chain release factor subunit 1 13 4 7 49032.6
Q99613 Eukaryotic translation initiation factor 3 subunit C 6 5 7 105347.2
O60841 Eukaryotic translation initiation factor 5B 12 11 24 138831.5
P02751 Fibronectin 8 10 21 262616.9
P09382 Galectin-1 39 5 11 14715.8
P14314 Glucosidase 2 subunit β 11 5 6 59425.8
P04406 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) 35 8 18 36053.4
P62826 GTP-binding nuclear protein Ran 28 5 10 24423.1
P11142 Heat shock cognate 71 kDa protein 13 5 16 70899.8
Q1KMD3 Heterogeneous nuclear ribonucleoprotein U-like protein 2 20 13 24 85105.2
Q9Y4L1 Hypoxia up-regulated protein 1 18 12 28 111336.8
P05556 Integrin β-1 16 10 20 88415.1
P05783 Keratin, type I cytoskeletal 18 53 21 61 48059.0
P35580 Myosin-10 8 11 25 229005.3
P35579 Myosin-9 25 38 96 226537.5
P07196 Neurofilament light polypeptide 53 25 69 61517.8
Q14978 Nucleolar and coiled-body phosphoprotein 1 8 5 10 73604.2
Q13823 Nucleolar GTP-binding protein 2 10 5 9 83656.0
Q9Y2X3 Nucleolar protein 58 21 8 17 59580.2
P19338 Nucleolin (C-23) 36 25 305 76615.9
P06748 Nucleophosmin 47 10 52 32575.5
Q99733 Nucleosome assembly protein 1-like 4 21 6 11 42823.9
P62937 Peptidyl-prolyl cis-trans isomerase A 28 5 9 18012.9
Q13427 Peptidyl-prolyl cis-trans isomerase G 5 4 6 88619.0
Q06830 Peroxiredoxin-1 59 9 18 22110.9
P18669 Phosphoglycerate mutase 1 25 4 6 28804.8
P13796 Plastin-2 15 6 10 70292.1
O00622 Protein CYR61 21 8 15 42026.0
P07237 Protein disulfide-isomerase 38 15 38 57118.1
P13667 Protein disulfide-isomerase A4 13 9 17 72934.0
Q58FF3 Putative endoplasmin-like protein 9 4 10 45859.7
Q58FF8 Putative heat shock protein HSP 90-β-2 18 6 11 44350.2
Q58FF7 Putative heat shock protein HSP 90-β-3 20 14 170 68326.5
P14618 Pyruvate kinase isozymes M1/M2 13 5 12 57937.5
P51149 Ras-related protein Rab-7a 33 6 10 23490.0
Q14692 Ribosome biogenesis protein BMS1 homolog 8 6 8 145812.2
Q14137 Ribosome biogenesis protein BOP1 23 12 20 83629.3
Q9Y265 RuvB-like 1 16 6 10 50229.4
P62136 Serine/threonine-protein phosphatase PP1-α catalytic subunit 25 6 9 37513.9
Q9BXP5 Serrate RNA effector molecule homolog 16 11 16 100669.7
Q9NQZ2 Something about silencing protein 10 19 10 35 54559.2
P78371 T-complex protein 1 subunit β 13 4 7 57489.9
P40227 T-complex protein 1 subunit ζ 17 4 7 58025.3
P37802 Transgelin-2 23 4 7 22391.9
P43307 Translocon-associated protein subunit α 20 4 9 32236.0
Q9BV38 WD repeat-containing protein 18 16 4 4 47405.1
Q86VM9 Zinc finger CCCH domain-containing protein 18 11 6 10 106379.8

P23396 40S ribosomal protein S3 35 7 14 DNA Damage Response/DNA Repair 26688.6
Q9Y5B9 FACT complex subunit SPT16 40 41 113 119917.4
Q08945 FACT complex subunit SSRP1 51 29 185 81077.6
P09429 High mobility group protein B1 (HMG B1) 23 5 22 24894.7
P26583 High mobility group protein B2 (HMG B2) 45 11 32 24034.6
P17096 High mobility group protein HMG-I/HMG-Y 45 5 10 11676.2
P16403 Histone H1D 19 5 14 21365.8
Q96QV6 Histone H2A type 1-A 40 5 18 14234.2
Q96A08 Histone H2B type 1-A 28 4 9 14168.0
P33778 Histone H2B type 1-B 36 8 31 13950.8
P62805 Histone H4 54 6 79 11367.7
P09874 Poly [ADP-ribose] polymerase 1 (PARP-1) 4 4 5 113087.8
Q9NY61 Protein AATF 41 17 37 63135.0
B2RPK0 Putative high mobility group protein B1-like 1 32 10 41 24238.8
P23246 Splicing factor, proline- and glutamine-rich 16 7 11 76149.5
Q9UIG0 Tyrosine-protein kinase BAZ1B 5 6 10 170907.0
P13010 X-ray repair cross-complementing protein 5 (XRCC-5 or Ku80/86) 11 4 10 82707.1
P12956 X-ray repair cross-complementing protein 6 (XRCC-6 or Ku70) 27 12 28 69846.4

Q15029 116 kDa U5 small nuclear ribonucleoprotein component 13 9 12 RNA Processing/mRNA Splicing 109438.1
P62081 40S ribosomal protein S7 32 4 8 22127.5
P62913 60S ribosomal protein L11 21 4 8 20253.2
Q08211 ATP-dependent RNA helicase A 8 7 13 140961.5
Q9NVP1 ATP-dependent RNA helicase DDX18 9 5 11 75409.7
O00148 ATP-dependent RNA helicase DDX39 9 4 6 49129.8
Q9H583 HEAT repeat-containing protein 1 3 5 8 242378.8
Q32P51 Heterogeneous nuclear ribonucleoprotein A1-like 2 27 7 17 34225.4
P51991 Heterogeneous nuclear ribonucleoprotein A3 25 8 21 39595.1
O60812 Heterogeneous nuclear ribonucleoprotein C-like 1 29 13 279 32142.7
P38159 Heterogeneous nuclear ribonucleoprotein G 22 9 19 42333.7
P31943 Heterogeneous nuclear ribonucleoprotein H 21 6 15 49229.8
P31942 Heterogeneous nuclear ribonucleoprotein H3 16 4 10 36927.6
P61978 Heterogeneous nuclear ribonucleoprotein K 41 12 24 50978.5
P14866 Heterogeneous nuclear ribonucleoprotein L 16 6 13 64132.8
P52272 Heterogeneous nuclear ribonucleoprotein M 16 9 18 77517.3
O60506 Heterogeneous nuclear ribonucleoprotein Q 15 9 15 69603.5
Q00839 Heterogeneous nuclear ribonucleoprotein U 37 29 78 90585.2
P22626 Heterogeneous nuclear ribonucleoproteins A2/B1 33 10 28 37430.3
P07910 Heterogeneous nuclear ribonucleoproteins C1/C2 48 13 739 33607.5
Q9UKD2 mRNA turnover protein 4 homolog 51 13 37 27561.3
P78316 Nucleolar protein 14 7 5 7 97671.9
O00567 Nucleolar protein 56 24 10 18 66052.0
Q9NR30 Nucleolar RNA helicase 2 14 9 13 87346.0
P12270 Nucleoprotein TPR 16 30 52 267289.3
O00541 Pescadillo homolog 36 19 55 68004.9
Q15365 Poly(rC)-binding protein 1 22 5 8 37498.2
P26599 Polypyrimidine tract-binding protein 1 30 9 20 57222.5
Q9HCG8 Pre-mRNA-splicing factor CWC22 homolog 4 4 11 105470.2
Q92841 Probable ATP-dependent RNA helicase DDX17 9 5 8 72373.0
P17844 Probable ATP-dependent RNA helicase DDX5 13 7 11 69149.7
P46087 Putative ribosomal RNA methyltransferase NOP2 26 18 35 89303.6
Q8IY81 Putative rRNA methyltransferase 3 34 19 40 96560.5
O76021 Ribosomal L1 domain-containing protein 1 18 7 12 54974.7
Q9NW13 RNA-binding protein 28 18 12 26 85739.1
Q9UKM9 RNA-binding protein Raly 37 11 22 32463.9
Q15287 RNA-binding protein with serine-rich domain 1 26 6 30 34209.6
Q9Y3B9 RRP15-like protein 17 5 9 31484.5
Q8IYB3 Serine/arginine repetitive matrix protein 1 8 4 7 102337.5
Q13435 Splicing factor 3B subunit 2 11 9 17 100229.4
Q07955 Splicing factor, arginine/serine-rich 1 39 9 16 27745.1
O75494 Splicing factor, arginine/serine-rich 10 23 5 10 31301.7
P84103 Splicing factor, arginine/serine-rich 3 29 5 11 19330.0
Q08170 Splicing factor, arginine/serine-rich 4 14 7 15 56680.0
Q13243 Splicing factor, arginine/serine-rich 5 19 5 12 31264.8
Q16629 Splicing factor, arginine/serine-rich 7 15 4 6 27367.5
P62995 Transformer-2 protein homolog beta 17 4 10 33666.7
P09661 U2 small nuclear ribonucleoprotein A′ 28 5 7 28417.1
O00566 U3 small nucleolar ribonucleoprotein protein MPP10 33 15 38 78866.8
O75643 U5 small nuclear ribonucleoprotein 200 kDa helicase 4 6 11 244513.8
Q96MU7 YTH domain-containing protein 1 9 7 12 84700.6

P62280 40S ribosomal protein S11 42 8 18 Transcriptional Regulation/Translation 18431.3
P62277 40S ribosomal protein S13 30 4 7 17223.3
P62263 40S ribosomal protein S14 36 4 9 16272.9
P62249 40S ribosomal protein S16 45 7 14 16445.9
P08708 40S ribosomal protein S17 55 5 14 15550.5
P62269 40S ribosomal protein S18 36 6 12 17719.3
P15880 40S ribosomal protein S2 23 6 10 31325.2
P62266 40S ribosomal protein S23 34 7 14 15807.7
P62701 40S ribosomal protein S4 21 5 8 29599.3
P46782 40S ribosomal protein S5 35 7 11 22877.0
P62241 40S ribosomal protein S8 42 7 11 24206.4
P46781 40S ribosomal protein S9 53 13 29 22592.5
Q8NHW5 60S acidic ribosomal protein P0-like 18 5 9 34365.1
Q96L21 60S ribosomal protein L10-like 21 5 14 24519.2
P30050 60S ribosomal protein L12 49 6 10 17819.1
P26373 60S ribosomal protein L13 33 7 11 24262.2
P40429 60S ribosomal protein L13a 33 12 26 23577.9
P50914 60S ribosomal protein L14 37 8 22 23432.3
P61313 60S ribosomal protein L15 39 8 18 24146.5
P18621 60S ribosomal protein L17 31 5 11 21397.4
Q07020 60S ribosomal protein L18 35 6 19 21635.2
Q02543 60S ribosomal protein L18a 52 10 23 20762.6
P84098 60S ribosomal protein L19 32 7 24 23467.4
P46778 60S ribosomal protein L21 34 5 12 18565.0
P62829 60S ribosomal protein L23 55 7 21 14865.9
P62750 60S ribosomal protein L23a 33 6 14 17696.2
P83731 60S ribosomal protein L24 41 7 15 17779.5
P61353 60S ribosomal protein L27 36 4 9 15798.4
P46776 60S ribosomal protein L27a 34 5 10 16561.4
P46779 60S ribosomal protein L28 49 8 16 15747.9
P39023 60S ribosomal protein L3 24 9 18 46109.5
P62888 60S ribosomal protein L30 51 4 9 12784.7
P62910 60S ribosomal protein L32 35 4 14 15860.4
P49207 60S ribosomal protein L34 29 5 9 13293.1
Q9Y3U8 60S ribosomal protein L36 35 5 12 12254.2
P61927 60S ribosomal protein L37 20 4 5 11078.2
P61513 60S ribosomal protein L37a 59 5 12 10275.4
P36578 60S ribosomal protein L4 36 13 28 47699.1
P46777 60S ribosomal protein L5 37 9 20 34363.5
Q02878 60S ribosomal protein L6 34 10 26 32729.3
P18124 60S ribosomal protein L7 40 10 22 29227.7
P62424 60S ribosomal protein L7a 40 9 22 29996.3
P62917 60S ribosomal protein L8 22 6 11 28024.8
P32969 60S ribosomal protein L9 55 6 17 21863.7
P11387 DNA topoisomerase 1 6 4 6 90729.7
Q03701 CCAAT/enhancer-binding protein ζ 11 8 14 120992.2
Q13185 Chromobox protein homolog 3 17 4 7 20812.0
O75367 Core histone macro-H2A.1 28 7 14 39618.9
O95602 DNA-directed RNA polymerase I subunit RPA1 4 4 6 194814.5
P68104 Elongation factor 1-α 1 (EF-1α1) 27 9 25 50141.2
P13639 Elongation factor 2 (EF-2) 17 10 24 95340.1
P60842 Eukaryotic initiation factor 4A-I 21 7 12 46155.3
P05198 Eukaryotic translation initiation factor 2 subunit 1 27 8 15 36112.7
P63241 Eukaryotic translation initiation factor 5A-1 39 4 8 16832.7
Q5SSJ5 Heterochromatin protein 1-binding protein 3 16 7 11 61208.7
Q99729 Heterogeneous nuclear ribonucleoprotein A/B 11 4 7 36225.2
O14979 Heterogeneous nuclear ribonucleoprotein D-like 15 4 8 46438.6
Q14103 Heterogeneous nuclear ribonucleoprotein D0 23 8 15 38434.5
P52926 High mobility group protein HMGA2 58 5 17 11831.9
Q12905 Interleukin enhancer-binding factor 2 62 16 80 43062.7
Q12906 Interleukin enhancer-binding factor 3 15 12 18 95338.9
P43243 Matrin-3 50 30 107 94626.7
Q9BQG0 Myb-binding protein 1A 28 31 80 148858.3
Q13765 Nascent polypeptide-associated complex subunit α 26 4 7 23383.3
P17480 Nucleolar transcription factor 1 20 14 33 89409.6
P55209 Nucleosome assembly protein 1-like 1 36 8 24 45375.0
Q9H307 Pinin 37 22 56 81613.2
P51531 Probable global transcription activator SNF2L2 9 10 22 181283.0
Q8IZL8 Proline-, glutamic acid- and leucine-rich protein 1 15 11 22 119700.6
P35659 Protein DEK 22 8 15 42675.9
Q8N7H5 RNA polymerase II-associated factor 1 homolog 15 6 12 59976.0
Q15424 Scaffold attachment factor B1 21 14 30 102642.8
Q92922 SWI/SNF complex subunit SMARCC1 22 18 42 122867.4
Q969G3 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 30 11 29 46649.7
Q9Y2W1 Thyroid hormone receptor-associated protein 3 13 12 33 108668.9
P51532 Transcription activator BRG1 11 14 28 184649.6
Q5BKZ1 Zinc finger protein 326 33 14 34 65653.5

Q9NVI7 ATPase family AAA domain-containing protein 3A 7 4 6 unknown 71370.2
P55081 Microfibrillar-associated protein 1 17 5 10 51958.7
Q9Y3T9 Nucleolar complex protein 2 homolog 30 19 70 84907.1
O94880 PHD finger protein 14 6 4 7 100055.1
Q96GQ7 Probable ATP-dependent RNA helicase DDX27 17 12 20 89838.1
Q9H0S4 Probable ATP-dependent RNA helicase DDX47 11 4 9 50648.4
Q9Y4W2 Protein LAS1 homolog 22 13 28 83065.5
Q9BXY0 Protein MAK16 homolog 50 12 30 35370.0
Q5JTH9 RRP12-like protein 5 4 6 143705.1
Q15061 WD repeat-containing protein 43 37 20 106 74890.8