Table 1.
List of primers used
| Gene of interest | Sequences of primers | Tm (°C) | References |
|---|---|---|---|
| PR-1b (basic PR1) | FOR 5′ CCAAGACTATCTTGCGGTTC 3′ | 55 | Van Kan et al. (1992) |
| REV 5′ GAACCTAAGCCACGATACCA3′ | |||
| PR2a (acidic glucanase) | FOR 5′ TATAGCCGTTGGAAACGAAG3′ | 55 | Van Kan et al. (1992) |
| REV 5′ TGATACTTTGGCCTCTGGTC3′ | |||
| PR2b (basic glucanase) | FOR 5′ CAACTTGCCATCACATTCTG 3′ | 52 | Van Kan et al. (1992) |
| REV 5′ CCAAAATGCTTCTCAAGCTC 3′ | |||
| PR 3a (chitinase 3, acidic) | FOR 5′ CAATTCGTTTCCAGGTTTTG 3′ | 52 | Danhash et al. (1993) |
| REV 5′ ACTTTCCGCTGCAGTATTTG3′ | |||
| PR 3b (chitinase 9, basic) | FOR 5′ AATTGTCAGAGCCAGTGTCC 3′ | 59 | Danhash et al. (1993) |
| REV 5′ TCCAAAAGACCTCTGATTGC 3′ | |||
| PR 5 (osmotin-like) | FOR 5′ AATTGCAATTTTAATGGTGC 3′ | 49 | Rep et al. (2002) |
| REV 5′ TAGCAGACCGTTTAAGATGC 3′ | |||
| PR 7 (P69A, subtulisin-like) | FOR 5′ AACTGCAGAACAAGTGAAGG 3′ | 50 | Tornero et al. (1996) |
| REV 5′ AAC GTGATTGTAGCAACAGG 3′ | |||
| PAL | FOR 5′ TTCAAGGCTACTCTGGC 3′ | 52 | Peng et al. (2014) |
| REV 5′ CAAGCCATTGTGGAGAT 3′ | |||
| Prot In (proteinase inhibitor) | FOR 5′ CGGAGAATCTGAATGGGTAAGCGA 3′ | 63 | Medeiros et al. (2009) |
| REV 5′ ACAAGCCGTGGTAAAGGTCCACAA 3′ | |||
| PO (peroxidase) | FOR 5′ ACGGAGCAAGCGACAATTGACAAC 3′ | 65 | Medeiros et al. (2009) |
| REV 5′ CGATTGATTCACCGCAAAGCTCGT 3′ | |||
| GST (glutathione-S-transferase) | FOR 5′ TGTCCCAACCTTCTCGTGCAGTTA 3′ | 65 | Medeiros et al. (2009) |
| REV 5′ TGAGTGATGCCAGTCCAACACAGA 3′ | |||
| Calmodulin | FOR 5′ GCACGGAAGATGAAGGACAC 3′ | 55 | Sanz-Alférez et al. (2008) |
| REV 5′ GCAAGCATCATACGGACAAAC 3′ | |||
| NR | FOR 5′ CAAGCAATCCATCTCCCAT 3′ | 57 | Jin et al. (2009) |
| REV 5′ CATCTCTGTATCGTCTTCAGGA 3′ | |||
| GAPDH (glyceraldehyde phosphate dehydrogenase) | FOR 5′ GAAATGCATCTTGCACTACCAACTGTCTTGC 3′ | 63 | Shih et al. (1992) |
| REV 5′ CTGTGAGTAACCCCATTCATTATCA TACCAAGC 3′ |