Table 3.
Quantitative analysis of phenolic acid content in tomato leaves due to foliar application of CaCl2 (0.5%), SNP (100 μM), C-PTIO (100 μM), L-NAME (10 μM) and pathogen inoculation alone or in different combinations
| Standard compounds | Quantity (µg/g of fresh weight) | |||||
|---|---|---|---|---|---|---|
| Control | Cacl2 | CaCl2 + L-NAME | CaCl2 + C-PTIO | SNP | CaCl2 + L-NAME + C-PTIO | |
| Gallic acid | NF | 25.84 ± 1.36b | NF | NF | 20.89 ± 0.59c | 4.62 ± 0.70d |
| Caffeic acid | 2.58 ± 0.30f | 67.95 ± 0.87c | 6.64 ± 0.79d | 3.77 ± 0.18ef | 80.03 ± 1.07a | 5.02 ± 0.42e |
| Vanillic acid | NF | 36.12 ± 0.86a | 12.36 ± 0.68c | 10.69 ± 0.72d | 27.22 ± 0.34b | NF |
| P-Coumaric acid | NF | 79.57 ± 2.48b | 26.03 ± 0.40f | 22.05 ± 0.36g | 59.86 ± 0.79c | 4.53 ± 0.17h |
| Ferulic acid | 2.67 ± 0.30h | 148.36 ± 3.95b | 55.91 ± 0.58d | 45.21 ± 0.60e | 111.28 ± 1.33c | 5.47 ± 1.07g |
| Myricetin | 6.74 ± 0.71d | 57.07 ± 1.81a | NF | NF | 14.81 ± 0.64c | NF |
| Quercetin | 4.92 ± 0.28f | 128.47 ± 2.0b | 15.02 ± 0.40d | 11.44 ± 0.87e | 130.47 ± 0.98a | 4.4 ± 0.32f |
| Cinnamic acid | 2.57 ± 0.28e | 71.17 ± 4.11b | 8.01 ± 0.68d | 4.79 ± 0.45e | 56.70 ± 0.86c | NF |
| Pyrogallol | 3.62 ± 0.31d | 7.88 ± 1.84b | 12.93 ± 0.71a | 9.06 ± 0.44b | 6.28 ± 0.36c | 4.88 ± 0.23d |
Values represent mean ± SE of three separate experiments, each in triplicate
CaCl 2 Calcium chloride, SNP sodium nitroprusside, C-PTIO 2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, L-NAME NG-nitro-l-arginine methyl ester, NF not found
Different letters within the row indicate significant difference (p < 0.05) from the control set using Duncan’s multiple range test. Same letter within the row denotes no significant difference between the groups